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Tetrazolium prepared in phosphate buffer
Where it is important to maintain the pH (6.5 - 7.5) for optimal activity, the tetrazolium (TZ) can be dissolved in a phosphate buffer. To prepare one litre:
Note: Any reagent that develops a pink colour should be discarded.
1. Seeds may be washed with distilled water.
Close-up of seeds of seeds of Orchis sancta showing dark red embryos of viable seeds. Photo Khaled Abulaila
Viable (deep red embryos) and non-viable (pale embryos) seeds of